Composite
Part:BBa_K4370002:Design
Designed by: Stéphanie Bury-Moné Group: iGEM22_GO_Paris-Saclay (2022-08-17)
RBS_cbbL_STR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 391
Design Notes
The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .
Source
This sequence has been synthetized.
References
Reference of Streptomyces bottropensis ATCC 25435 genome: GCF_000383595.1_ASM38359v1